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李明成, 王放, 李凡. 食品中变形杆菌第1类整合子鉴定和特征[J]. 中国公共卫生, 2006, 22(1): 29-30. DOI: 10.11847/zgggws2006-22-01-16
引用本文: 李明成, 王放, 李凡. 食品中变形杆菌第1类整合子鉴定和特征[J]. 中国公共卫生, 2006, 22(1): 29-30. DOI: 10.11847/zgggws2006-22-01-16
LI Mingcheng, WANG Fang, LI Fan. Identification and characterization of class 1 integron of Proteuse in meats food[J]. Chinese Journal of Public Health, 2006, 22(1): 29-30. DOI: 10.11847/zgggws2006-22-01-16
Citation: LI Mingcheng, WANG Fang, LI Fan. Identification and characterization of class 1 integron of Proteuse in meats food[J]. Chinese Journal of Public Health, 2006, 22(1): 29-30. DOI: 10.11847/zgggws2006-22-01-16

食品中变形杆菌第1类整合子鉴定和特征

Identification and characterization of class 1 integron of Proteuse in meats food

  • 摘要:
      目的   探讨肉类食品中变形杆菌第1类整合子的分布、特征及所含基因盒的种类.
      方法   常规方法分离变形杆菌; 纸片扩散法对9种抗生素进行耐药性监测和分析; PCR鉴定1类整合子; 阳性株PCR产物测序并对结果进行分析.
      结果   91份标本中共鉴定出27株变形杆菌, 有13株(48.1%)对至少4种以上抗生素表现多重耐药, 耐药谱为复合磺胺-氨苄西林-红霉素-四环素-链霉素.13株中有3株鉴定出1类整合子, 2株携带0.75和1.8 kb 2个整合子.1株携带1.0 kb整合子, PCR产物测序, 1.0 kb整合子携带aadA2基因盒, 传递对氨基糖苷类抗生素的耐药性.
      结论   肉类食品中变形杆菌携带第1类整合子, 1.8 kb整合子携带pse-1-aadA1-dfrA14基金盒, 传递对β-内酰胺类、磺胺类、氨基糖苷类抗生素的耐药性.携带复杂的基因盒, 决定着对β-内酰胺类、氨基糖苷类和磺胺类抗生素的耐药性.

     

    Abstract:
      Objective   To explore the distribution and character of class 1 integrons in Porteues from meats, and to elucidate the status of gene-cassettes.
      Methods   Routine method was used to isolate Proteues and antibiotics susceptibility was tested by the disk diffusion method; class 1 integron was detected by PCR assay; PCR products were sequenced and analyzed.
      Results   Of 91 samples, 27 isolates were identified, and 13 isolates were multiple-drug resistant.The antibiogram was sulfamethoxazole-trimethoprim, ampicillin, streptomycin, tetracycline, erythromycin.3 of 14 isolates carried class 1 integrons; and two strains laboured two integrons with 1.8 kb and 0.75 kb, one strain laboured 110 kb integron.The sequenced PCR conduct demonstrated that the 118 kb integron laboured pse-1-aadA1-dfrA14 gene cassettes confered the resistance of β-lactamas, sulfamethoxazole-trimethoprim, and aminoglycoside; the 110 kb integron laboured aadA1 gene cassette confered the resistance of aminoglycoside.
      Conclusion   The different kinds of class 1 integrons exists in proteues from meats, and determines the multiple-resistant antibiotics.

     

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