沙棘油和亚硒酸钠对汞中毒大鼠的拮抗作用

王家骏; 徐兆发; 贺安宁; 杨敬华; 徐斌

doi:10.11847/zgggws2006-22-03-21

沙棘油和亚硒酸钠对汞中毒大鼠的拮抗作用

Antagonistic effects of sea buckthron oil and Na2SeO3 on rats exposed to mercuric chloride

摘要

摘要:
目的探讨在体一次染毒条件下氯化汞对大鼠肝肾脏毒性作用并观察沙棘油和亚硒酸钠预处理对汞毒性的影响。
方法将Wistar大鼠随机分成对照组、单纯染汞组、沙棘油和亚硒酸钠干预组。各组经染毒处理12 h后收集大鼠12 h尿液, 采集血液、切取肝脏和肾皮质样品。测定肝脏、肾皮质和尿汞含量; 尿N-乙酰--βD-氨基葡萄苷酶(NAG)、碱性磷酸酶(ALP)、乳酸脱氢酶(LDH)活性和尿蛋白、血清尿素氮(BUN)含量。
结果沙棘油干预组肝汞、肾皮质汞、尿NAG、尿ALP活性和BUN含量显著低于单纯染汞组。亚硒酸钠干预组肝汞含量显著高于单纯染汞组。而肾皮质汞、尿汞含量和尿NAG、ALP、LDH活性、尿蛋白、BUN含量则显著低于单纯染汞组。
结论沙棘油和亚硒酸钠对汞的肝、肾脏毒性具有一定拮抗作用。

Abstract:
Objective To study the liver and renal toxicity induced by mercury and to observe the effects of Seabuckthom seed oil(SBO), Na ₂SeO ₃ pretreatment on the liverotoxicity and nephrotoxicity of mercury.
Methods Wistar rats were divided randomly into control group, mercury group and SBO, Na ₂SeO ₃ pretreatment groups.The 12h urine samples and the blood samples were collected, The liver and renal cortex were also removed.Mercury contents in the liver, renal cortex and urine samples were measured.Urinary Nacety-l B-D-glucosaminidase(NAG), alkaline phosphates(ALP), lactose dehydrogenase(LDH)activities, urinary protein and serum urea nitrogen(BUN)contents were also determined.
Results Mercury contents of urine of SBO pretreatment group were higher significantly than that of 2.5 mg/kg HgCl ₂ group.Mercury contents in the liver of Na ₂SeO ₃ pretreatment group were higher than that of 2.5 mg/kg HgCl ₂ group, Mercury concentrations in renal cortex and urine, Urinary NAG, ALP, LDH activities, urinary protein and BUN contents were lower significantly than that of 2.5 mg/kg HgCl ₂ group.
Conclusion SBO and Na ₂SeO ₃ had antagonistic effects on liverotoxicity and nephrotoxicity of mercurty.

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