铅对脐带血造血细胞增殖影响

邱亿腾; 邹文霞; 钟义富; 李容汉; 黄健红

doi:10.11847/zgggws2006-22-03-27

铅对脐带血造血细胞增殖影响

Effects of lead on proliferation of hematopoietic cells in umblical cord blood

摘要

摘要:
目的探讨铅对造血细胞增殖分化的影响。
方法采集脐血21份, 同时检测脐血中铅水平以及采用Fi-coll-Hypaque淋巴细胞分离液离心获取单个核细胞, 在体外悬浮培养15 d, 定期观察细胞增值情况。
结果脐铅含量平均为(54.3±1.7)μg/L。从细胞生长密度随培养时间变化的趋势看, 不同铅水平的细胞生长密度差异有统计学意义。高铅水平的单个核细胞群的扩增能力明显低于低铅水平组。铅对单个核细胞群增值和分化的抑制作用主要表现于培养初期, 对于培养后期的细胞群, 其抑制作用明显减弱。
结论铅对各阶段造血细胞增和分化的抑制作用不同, 其机制有待进一步研究。

Abstract:
Objective To observe the effects of lead on proliferation of hematopoietic cells in umblical cord blood(UCB).
Methods The concentration of lead was detected in 21 UCBs.After being harvested by centrifugation with Ficoll Hypaque lymphocyte separating medium, the mononuclear cells(MNCs)were cultured in vitro for 15 days and their proliferation was observed periodically.
Results The concentration of lead in UCB was(54.3±1.7)μg/L.The difference of cell density between various lead content was significant.The amplification of MNCs with high lead level was inferior to that with low lead level.The inhibition of lead from proliferation and differentiation of MNCs was resented mainly in the early days during culture and the effect was weakened at the later stage.
Conclusion The effect of lead on proliferation and differentiation of UCB hematopoietic cells is dissimilar at various stages, the mechanism of which should be further studied.

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