雌激素受体基因多态性与男性骨密度关系

吴涤; 洪秀梅; 蒋英俊; 李志平; 臧桐华

doi:10.11847/zgggws2006-22-03-35

雌激素受体基因多态性与男性骨密度关系

Study on association of estrogen receptor α gene polymorphisms with bone mineral density in Chinese men

摘要

摘要:
目的研究雌激素受体(ER)α基因多态性与中国男性骨密度关系。
方法采用双能X线吸收仪对339名男性研究对象进行腰椎及股骨扫描, 应用PCR-限制性片段长度多态性(PCR-RFLP)方法检测ER-α基因PvuⅡ和XbaⅠ两多态位点基因型, 用广义线性模型分析两位点多态性与腰椎及股骨骨密度关系。
结果在调整环境危险因素后, 显示ER基因的XX基因型与股骨柄、股骨三角区的骨密度增高呈显著相关, 差异有统计学意义(P值分别为0.004和0.036);Pp基因型与股骨柄骨密度增高有相关趋势, 但差异无统计学意义(P=0.064);XP单倍型与股骨柄骨密度增高有相关的趋势, 但差异无统计学意义(P=0.053)。
结论 ER-α基因XbaⅠ位点多态性与男性股骨骨密度相关。PvuⅡ位点多态性和XP单倍型与股骨骨密度关系还需进一步扩大样本量研究。

Abstract:
Objective To investigate the association of polymorphisms of the estrogen receptor A(ER-A)gene with bone mineral density(BMD)in Chinese men.
Methods The bone mineral density was measured at the lumbar spine and femur using dual-energy X-ray absorptiometry in 339 study subjects.PCR-restriction fragment length polymorphism(PCR-RFLP)was applied to examine the Pvu Ⅱ and Xba Ⅰ polymorphisms of the ER-Agene.Generalized linear modelwas used to analysis the association of polymorphisms in two sites with the bone mineral density of the lumbar spine and femur.
Results After adjustment for environmental covariates, signifwamt correlation was found between XX genotype of the ER-A gene and higher BMD of Femoral shaft and Ward triangle, had reached statistically significant(P=0.004 and P=0.036, respectively); A tendency of higher BMD at femoral shaft with Pp genotype population was observed, but not reached statistically significant(P=0.064).Haplotype XP tended to be associated with higher BMD of femoral shaft, but not reached statisligally significant(P=0.053).
Conclusion There is significant relationship between XbaⅠ polymorphisms of ER-α gene and femur BMD in Chinese men.The correlation between polymorphism of PvuⅡ site and haplotype XP with BMD of femur are needed study at larger samples.

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