超抗原SEB诱导外周血单核细胞杀伤肿瘤效果

Study on tumor-cytotoxicity effect of PBMC induced by super antigen SEB

摘要:
目的通过观察葡萄球菌肠毒素B(SEB)对外周血单核细胞(PBMC)杀瘤活性的影响, 寻找适用于肿瘤过继免疫治疗的新途径。
方法密度梯度离心法分离富集PBMC, 分别用10^-12, 10^-11, 10^-10, 10^-9g/L浓度的SEB作用于PBMC, 12, 24, 36, 48 h后测定PBMC对K562细胞的杀伤活性。
结果 SEB处理PBMC 12 h后杀瘤活性增强, 至24 h杀瘤活性最佳; 在一定浓度范围内PBMC的杀瘤活性随SEB浓度的升高而增强。
结论超抗原SEB在体外能够增强PBMC的杀瘤活性。

Abstract:
Objective Observing the tumor-cytotoxicity effect of periphera blood mononuclear cell(PBMC)induced by super antigen staphylococcus enterotoxin B(SEB), so to find a new method in tumor adoptive immunotherapy.
Methods Density grads centrifuge was used to separate and rich PBMC, then PBMCs were stimulated with different concentration of SEB, 10^-12 g/L, 10^-11 g/L, 10^-10 g/L and 10^-9 g/L.The tumor-cytotoxicity effect of k562 was detected after being induced 12, 24, 36 and 48 h.
Results The tumo-rcytotoxicity effect of PBMC began to increase 12 h later, and the optimum time was 24 h.The tumo-rcytotoxicity effect of PBMC is increasing with risement of SEB in a certain content.
Conclusion Super antigen SEB does enhance the tumor-cytotoxicity of PBMC in vitro.