茶多酚对人肝癌细胞激活蛋白1及细胞周期影响

Effect of tea polyphenols on cell cycle of human liver cancer cell

摘要:
目的观察茶多酚对人肝癌细胞激活蛋白1(AP-1)及细胞周期的影响。
方法构建AP-1报告质粒测量AP-1活性, 免疫组化测量细胞周期素D₁和细胞周期素依赖激酶4表达变化, 流式细胞仪分析细胞周期。
结果茶多酚抑制AP-1活性, 细胞周期素D₁和细胞周期素依赖激酶4表达降低, 细胞阻滞在G₁期。
结论茶多酚可能通过下调AP-1活性使细胞周期素D₁表达降低; 而茶多酚使细胞周期素依赖激酶4表达降低, 可能与AP-1的活性无关。

Abstract:
Objective To study the effects of tea polyphenols on activator protein 1 and cell cycle of human liver cancer cell.
Methods An activator protein 1 luciferase reporter gene was constructed and the activities of activator protein 1 were measured.The expression changes of cyclin D₁ and cyclin-denpendent kinase 4 were studied by the immunity organization chemistry method.cell cycle was analyzed by flow cytometry.
Results The activities of activator protein 1 were repressed, the experession of cyclin D₁ and cyclin-denpendent kinase 4 was reduced, G₁ arrest was induced by tea polyphenols in cell line.
Conclusion Tea polyphenols possibly repressed the expression of cyclin D₁ through down-regulating the activities of activator protein 1;It had nothing to activities of activator protein 1 that tea polyphenols repressed the expression of cyclin-denpendent kinase 4.