丙烯腈对鼠胚胎脊髓神经细胞增殖分化的影响

端礼荣; 邢光伟; 王卉芳

doi:10.11847/zgggws2006-22-03-43

丙烯腈对鼠胚胎脊髓神经细胞增殖分化的影响

Effect of acrylonitrile on proliferation and differentiation of rat embryo spinal cord nerve cell

摘要

摘要:
目的研究丙烯腈(ACN)对大鼠胚胎脊髓神经细胞增殖分化的影响。
方法 16 d的大鼠胚胎脊髓组织经取材、分离、消化后作原代细胞培养, 加入不同浓度的ACN 0.01, 0.1, 1.0, 10.0, 50.0, 100.0, 200.0μg/ml, 从细胞形态学及细胞计数角度观察细胞生长与分化过程, 同时测定蛋白质含量、丙二醛(MDA)含量和超氧化物歧化酶(SOD)活性并与对照组进行比较。
结果各组ACN明显抑制胚胎脊髓神经细胞的增殖和分化, 集落形成率明显减少, 细胞体积小; 其半数分化抑制剂量(ICD₅₀)为29μg/ml, 半数存活抑制剂量(ICV₅₀)为42μg/ml, 均显示剂量-效应关系。
结论 ACN能抑制胚胎脊髓神经细胞增殖和分化, 可能与其能抑制蛋白质合成, 并引起脂质过氧化有关。

Abstract:
Objective To observe the effect on spinal nerve cell of rat embryo by plating acrylonitrile(ACN).
Methods ACN solution(0.01, 0.1, 1.0, 10.0, 50.0, 100.0, 200.0 μg/ml)were put into primary spinal nerve cell of rat embryo of dawing been cultured 16 days; the concentration rates were conrted; the content of protein of malondialdehyde(MDA)and activity of superoxide dismutase(SOD)were determined.
Results Acrylintrile inhibited differentiation and proliferation spinal nerve cell of rat embryo.The concentration rate and dimension of cell were reduced.The concenration of 50% inhibitions of diffferentiation(ICD₅₀)was 29μg/ml.The concentration of 50% inhibitions of cells proliferation(ICV₅₀)was 42 μg/mL.
Conclusion The inhibition effect of acrylonitrile on spinal nerve cell of rat embryo might be associated with impact of protein synthetis and with causing lipid peroxidation.

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