激素类除草剂二氯喹啉酸遗传毒性比较

Comparative research on genotoxicity of auxin herbicide quinclorac

摘要:
目的探讨激素类除草剂二氯喹啉酸的遗传毒性。
方法应用鼠伤寒沙门菌体外回复突变试验(Amestest)和彗星实验(Comet assay)对激素类除草剂二氯喹啉酸的遗传毒性进行比较研究。
结果 Ames试验中TA97, TA98, TA100菌株无论代谢活化或非代谢活化, 皆为阳性结果, 而TA102菌株则为阴性结果。彗星实验在500, 1 000, 2 000 mg/(kg·bw)浓度下, 二氯喹啉酸对小鼠骨髓细胞的DNA损伤与阴性对照组比较差异有统计学意义(P < 0.01), 且呈剂量-效应关系。
结论激素类除草剂二氯喹啉酸具有一定的遗传毒性。

Abstract:
Objective To study the genotoxicity of auxin herbicide quinclorac.
Methods The Ames test and Comet Assay were used to detect the genotox icity of auxin herbicide quinclorac.
Results With and without S9 activation, there was positive result of the TA97, TA98 and TA100 strains and negative result of the TA102 strain in Ames test.Compared with the control group, the DNA migration increased significantly at the different three test doses of Erythroblast cells(P < 0.01)and exhibited a significant dose-effect relationship.
Conclusion The aux in herbicide quinclorac could cause genotoxicity.