镉诱导LLC-PK<sub>1</sub>细胞凋亡对基因表达的影响

任香梅; 蔡云清; 吴小丽

doi:10.11847/zgggws2006-22-06-27

镉诱导LLC-PK₁细胞凋亡对基因表达的影响

Effect of cadmium on apoptosis and expression of bcl-2, p53, c-myc, c-fos and ras genes in LLC-PK₁ cells

摘要

摘要:
目的探讨镉诱导猪肾近曲小管上皮细胞(LLC-PK₁)凋亡对bcl-2、p53(mtp53)、c-mycc、-fos与ras基因表达的影响.
方法采用流式细胞仪分别测定bcl-2、p53、c-myc、c-fos与ras基因表达产物.
结果不同浓度CdCl2(0, 10, 20, 40μmol/L)作用LLC-PK₁细胞, 8 h后bcl-2表达逐渐下降, 并呈良好的剂量-反应关系(r=-0.910, P < 0.05);4, 8 h p53表达均明显下降, 呈剂量-反应关系(r值分别为-0.716, -0.972, P均 < 0.05);8 h后c-myc表达逐渐下降, 呈剂量-反应关系(r=-0.694, P < 0.05);8 h后c-fos表达未见剂量-反应关系; 8 h后ras表达逐渐下降, 呈明显的剂量-反应关系(r=-0.887, P < 0.05).
结论镉诱导LLC-PK₁细胞凋亡的机制可能与镉抑制bcl-2、p53、c-mycr、as基因表达有关.

Abstract:
Objective To explore the effect of cadmium on the apoptosis of porcine renal prox imal tubule epithelial cells(LLC-PK₁)and the relationship between the apo ptosis and the bc-l 2, p53, -cmy c, c-fos and ras gene expression.
Methods The expression of bc-l 2, p53, c-myc, c-fos and ras were measured respectively by FCM.
Results After LLC-PK₁ cells were incubated with 0, 10, 20, 40 Lmol/LCdCl2 for4 h or8 h, the expression of bc-l 2, p53, c-myc and ras decreased and there was a significant dos-eresponse relationship(r=-0.910 of bc-l2 for 8 h, r=-0.716, -0.972 of p53 for4 h, 8h respectively, r=-0.694 of c-myc for 8h and r=-0.887 of ras for 8 h).
Conclusion The apoptosis of LLC-PK₁ cells could be induced by cadmium, and the inhibition of the expression of bc-l 2, p53, c-myc and ras would be one possible mechanism.

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镉诱导LLC-PK1细胞凋亡对基因表达的影响

Effect of cadmium on apoptosis and expression of bcl-2, p53, c-myc, c-fos and ras genes in LLC-PK1 cells

镉诱导LLC-PK₁细胞凋亡对基因表达的影响

Effect of cadmium on apoptosis and expression of bcl-2, p53, c-myc, c-fos and ras genes in LLC-PK₁ cells