碱性成纤维细胞生长因子对卵巢癌的影响

刘萍; 李新鸣; 孙黎光; 文涛; 侯伟健; 于卉影; 赵晓光

doi:10.11847/zgggws2006-22-06-40

碱性成纤维细胞生长因子对卵巢癌的影响

Effects of basic fibroblast growth factor on proliferation and apoptosis in ovarian cancer cell CAOV3 mediated by ERK1/2

摘要

摘要:
目的观察Ras-Raf-ERK 1/2途径介导的碱性成纤维细胞生长因子(bFGF)对卵巢癌细胞系CAOV3细胞增殖和凋亡影响, 探讨bFGF及其信号转导途径与卵巢癌发生发展关系.
方法以bFGF和促细胞分裂剂激活性蛋白激酶1(MEK1)抑制剂PD98059处理CAOV3细胞, 用四甲基偶氮噻唑蓝(MTT)法检测细胞增殖情况, 流式细胞术检测细胞凋亡情况, 蛋白印迹(Western blot)检测细胞外信号调节蛋白激酶1/2(ERK1/2)的活性.
结果 bFGF处理后细胞增殖比明显增加, 且与bFGF水平呈剂量依赖关系, bFGF浓度为75 ng/ml时细胞增殖比最高为140%;bFGF使无血清诱导的凋亡细胞比例下降(33.20±5.32)%2.38±3.36%; bFGF诱导ERK1/2活性增高.PD98059可抑制bFGF的这些作用.
结论 bFGF通过Ras-Raf-ERK 1/2途径介导, 促进卵巢癌CAOV3细胞增殖, 抵抗无血清诱导凋亡.在卵巢癌发生发展过程中, bFGF信号传递发挥了重要作用.

Abstract:
Objective To investigate Ras-Raf-ERK 1/2 mediated functions of basic fibroblast growth factor(bFGF) on proliferation and apoptosis in ovarian cancercell CAOV 3 and to study the relationship between signal transduction mechanism of bFGFand the development of ovarian cancer.
Methods Aftertreated with bFGFand PD98059, the inhibitorof mitogen-activ ated protein kinase(MEK 1), proliferation rate, apoptosis rate, ext racelluarsignal-regulated protein kinase(ERK1/2)activity of CAOV 3 cell were studied by means of methyl thiazoly l tetrazolian(MT T)assay, FACScan flowcy tometer, Western blot respectively.
Results bFGFcould dose dependently improve the proliferation of CAOV 3 cell significantly.Treated cells with 75 ng/ml bFGF, the pro liferation rate increased to 140%: bFGFdecreased the apoptosis rate induced by starvation(33.20±5.32)% ~2.38±3.36)%; bFGFinduced ERK 1/2 activity.All these effects of bFGFwere inhibited by PD98059.
Conclusion In ovarian cancercell CAOV3, bFGFincreased pro liferation rate, decreased apoptosis rate, which was mediated by Ras-Raf-ERK1/2 pathway.The bFGFsignal transduction might play important roles in the development of ovarian cancer.

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