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王丽, 乔琰, 鲁志松, 丁书茂, 严彦, 杨旭. 亚急性甲醛吸入对小鼠肝细胞DNA损伤作用[J]. 中国公共卫生, 2006, 22(6): 709-710. DOI: 10.11847/zgggws2006-22-06-45
引用本文: 王丽, 乔琰, 鲁志松, 丁书茂, 严彦, 杨旭. 亚急性甲醛吸入对小鼠肝细胞DNA损伤作用[J]. 中国公共卫生, 2006, 22(6): 709-710. DOI: 10.11847/zgggws2006-22-06-45
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WANG Li, QIAO Yan, LU Zhisong, . Damage effect of subacute formaldehyde on hepatocytic DNA in mice[J]. Chinese Journal of Public Health, 2006, 22(6): 709-710. DOI: 10.11847/zgggws2006-22-06-45
Citation: WANG Li, QIAO Yan, LU Zhisong, . Damage effect of subacute formaldehyde on hepatocytic DNA in mice[J]. Chinese Journal of Public Health, 2006, 22(6): 709-710. DOI: 10.11847/zgggws2006-22-06-45
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亚急性甲醛吸入对小鼠肝细胞DNA损伤作用

Damage effect of subacute formaldehyde on hepatocytic DNA in mice

    摘要
  • 摘要:
      目的   检测亚急性甲醛吸入对小鼠肝细胞的损伤作用, 并探讨用归一化处理方法解决单细胞凝胶电泳重复性差的可行性.
      方法   将小鼠分别暴露于不同浓度的气态甲醛(0.5, 1.0和3.0 mg/m3)中染毒14 d, 每天6 h, 用单细胞凝胶电泳技术检测肝细胞DNA的损伤程度.
      结果   0.5和1.0 mg/m3的甲醛可造成DNA的严重断裂(P < 0.001), 而3.0 mg/m3的甲醛则引起显著的交联作用, 归一化的结果与之相符, 并表明不同浓度的甲醛导致不同程度和类型的DNA损伤.
      结论   甲醛可造成机体内肝细胞DNA的断裂和交联, 该损伤可能是甲醛致癌机制之一; 归一化可以弥补单细胞凝胶电泳实验重复性不佳的缺陷.

     

    Abstract:
      Objective   To explore the hepatocellular DNA damage aftersubacute formaldehyde exposure and the application of normalization as a statistic method to improve the reproducibility of single cell gel electrophoresis(SCGE, comet assay).
      Methods   Mice were respectively exposed to different concentrations of gaseous formaldehyde for 14 d, 6 h perday.SCGEwas performed to detect the DNAdamages.And normalization was used to discuss the reproducibility of SCGEassay.
      Results   Formaldehyde could induce different kinds of DNAdamages: formaldehyde caused significant DNAstrands breaks at concentrations of 0.5 and 1.0 mg/m3(P < 0.001)while crosslinks were induced at 3.0 mg/m3.
      Results   from normalization were in consistent with the formerresults and indicated that DNAdamages were enhanced with the increase of formaldehyde level.
      Conclusion   Formaldehyde was both a DNAstrands breaks and crosslinks agent to the hepatocytes in vivo, which may be one of the possible mechanisms of formaldehyde as a carcinogen.Normalization could improve the reproducibility of SCGEassay.

     

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