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高军, 杨桦, 郑兰艳, 鲁润铭, 牟玲, 戴文影. 肾综合征出血热病毒核酸的免疫研究[J]. 中国公共卫生, 2006, 22(6): 718-718. DOI: 10.11847/zgggws2006-22-06-53
引用本文: 高军, 杨桦, 郑兰艳, 鲁润铭, 牟玲, 戴文影. 肾综合征出血热病毒核酸的免疫研究[J]. 中国公共卫生, 2006, 22(6): 718-718. DOI: 10.11847/zgggws2006-22-06-53
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GAO Jun, YANG Hua, ZHENG Lanyang, . Study on neucleic acid immunity of hemorrhagic fever renal syndrome virus[J]. Chinese Journal of Public Health, 2006, 22(6): 718-718. DOI: 10.11847/zgggws2006-22-06-53
Citation: GAO Jun, YANG Hua, ZHENG Lanyang, . Study on neucleic acid immunity of hemorrhagic fever renal syndrome virus[J]. Chinese Journal of Public Health, 2006, 22(6): 718-718. DOI: 10.11847/zgggws2006-22-06-53
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肾综合征出血热病毒核酸的免疫研究

Study on neucleic acid immunity of hemorrhagic fever renal syndrome virus

    摘要
  • 摘要:
      目的   编码NP的S基因进行病毒核酸的免疫研究.
      方法   进行实验动物的DNA免疫, 细胞因子的测定, 淋巴细胞增殖反应测定, 免疫鼠血清的抗核蛋白NP抗体的测定.
      结果   蛋白在Vero-E6细胞中的瞬时表达.免疫鼠细胞因子: 接种pcDNA3.1+S的鼠IL-4的产生和IFN-γ的产生均较对照组要高.细胞增殖反应: 阳性对照刀豆蛋白A(ConA)刺激组的增殖指数为2.1, 对照空载体增殖指数较低为0.797, 而实验组pcDNA3.1+S接种组和pcDNA3.1+S(ISS)接种组的增殖指数分别为1.43和1.67.免疫鼠血清特异性抗体的检测: 接种pcDNA3.1+S(ISS)免疫鼠的血清抗体水平较接种pcDNA3.1+S免疫鼠的血清抗体水平升高明显.而空载体pcDNA3.1+接种组的小鼠血清抗体水平低.
      结论   肾综合征出血热(HFRS)病毒编码NP的S基因作为病原体的抗原基因进行的核酸免疫, 可以起到免疫保护作用.

     

    Abstract:
      Objective   To study neucleic acid immunity of Sgene of NP, which coded by hemorrhagic feverrenal sy ndrome virus(HFRSV).
      Methods   (1) DNAimmunity of experimental animal.(2)Examination of cytokines.(3)Ex amination of proliferation reaction of lymphocyte.(4)Examination of anti-NP Ab in immune rats'serum.
      Results   (1) Instantaneous expression of protein in Vero-E6 cells.(2)Cytokine in immune rats: products of IL-4 and IFN-g were more in rats thathad been inoculated by pcDNA 3.1+Sthan those in control group.IL-4 in pcDNA3.1+S(ISS)immuned rats was athigh level.(3)Cell proliferation reaction: The proliferation index was 2.1 in con-Astimulated positive control group and 0.797 of spleno cyte in plaque carrierpcDNA3.1+inoculated control group while it were 1.43 and 1.67 respectively in pcDNA3.1+Sinoculated experimental group and pcDNA3.1+S(ISS)ino culated group.(4)Examination of specific Ab in immune rats serum: The Ab level in pcDNA3.1+S(ISS)inoculated immune rats serum was distinctly higherthan thatin pcDNA3.1+Sinoculated immune rats.But the serum Ab in plaque carrierpcDNA 3.1+inoculated group waved at lowerlevel continuously.
      Conclusion   Neucleic acid immunity of Sgene of NP, co ded by HFRSV, as a pathogenic and antigenic gene, have protection of immunity.

     

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