依布哂啉和谷胱甘肽对大鼠过氧化抑制作用

林凯; 鲁文清; 张红宇; 张静

doi:10.11847/zgggws2006-22-07-47

依布哂啉和谷胱甘肽对大鼠过氧化抑制作用

Inhibitory effect of ebselen and GSH on peroxidation in rats

摘要

摘要:
目的探索依布硒啉(Ebs)和谷胱甘肽(GSH)对酒精依赖大鼠过氧化损伤的抑制作用和效果。
方法测定第3周末酒精依赖大鼠血清中的谷胱甘肽过氧化物酶(GPx)活性和脂质过氧化物丙二醛(MDA)生成量, 以GPx与MDA比值反映过氧化抑制作用。将服药组的GPx与MDA比值减去阳性组GPx与MDA比值除以服药组GPx与MDA比值的百分率, 得出抑制效率。
结果 Ebs 0.1~10 mg/kg与GSH5~10 mg/kg联合作用的过氧化抑制效率在40.0%~65.7%之间(P_均 < 0.01), 显示剂量与功效关系(r=0.693, P < 0.01)。Ebs 5~10 mg/kg的抑制效率为36.7%~45.3%。
结论酒精过氧化损伤时的Ebs抗氧化作用除依赖辅助因子GSH外, 还可能通过其他途径产生。

Abstract:
Objective To inv estigate inhibitory effect of Ebselen and glutathione(GSH)on perox idation of Alcohol-dependent rats.
Methods Serum glutathione peroxidase(GPx)activity and lipid peroxide(LPO)content and malo ndialdehyde (MDA)of alcohol-dependent rats were detected in end of 3 weeks afterexposure, and ratio of GPx activity to MDA was applied to index the inhibitory effect of peroxidation.The ratio of each ebselen-exposure group with different concentration substracted the ratio of positive control group, the result was divided by the ratio of ebselen-exposure group and a percentage was gotten which represented the efficiency of inhibition.
Results Ebselen and GSH were administerd simultaneously and a synergistic effect could be invesgated when the concentration of Ebselen and GSH varied from 0.5mg/kg to 10mg/kg and 5~10mg/kg, respectively.The efficiency of inhibition increased from 40.0% to 65.7% (P_ar < 0.01), which displayed a dose-response relationship(r=0.693, P < 0.01).When ebselen of different concentration(5~10 mg/kg)was administered singly, the inhibitory effect could also be obtainde which varied from 36.7% to 45.3%.
Conclusion Ebselen can display antiox idative effect with the co-factor GSH, and other pathways may also exertin the mechanism of antiox idant effect of ebselen.

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