杀螟硫磷体外环境雌激素活性及机制研究

富英群; 单晓英; 李国强; 李百祥

doi:10.11847/zgggws2006-22-07-48

杀螟硫磷体外环境雌激素活性及机制研究

Study on environmental estrogenic activity of fenitrothion in vitro and its mechanism

摘要

摘要:
目的检测杀螟硫磷的环境雌激素活性, 并探讨其作用机制。
方法人乳腺癌细胞株(MCF-7)细胞在含10%小牛血清的RPMI1640培养基中进行半开放式贴壁培养, 加受试物前2周, 改在含10%去激素小牛血清的无酚红RPMI1640培养基中培养。试验设溶剂对照组、阳性对照组及杀螟硫磷5个不同浓度实验组, 采用MCF-7细胞增殖试验、四甲基偶氮噻唑蓝(MTT)试验和生长曲线观察杀螟硫磷对MCF-7细胞增殖的影响, 并通过雌激素受体(ER)结合试验分析杀螟硫磷与ER的结合情况。
结果杀螟硫磷在10^-9~10^-6mol/L剂量范围内可诱导MCF-7细胞增殖, 差异有统计学意义(P < 0.05), 且存在剂量-效应关系。杀螟硫磷能与ER结合, 抑制125I-E2与ER结合。
结论杀螟硫磷具有环境雌激素活性, 其拟雌激素作用由ER介导而产生。

Abstract:
Objective To evaluate the env ironmental estrog enic activit y of the pesticide fenitr othion and study the possible mechanism.
Methods Human breast cancer cell strain MCF-7 cells were maintained in RPMI 1640 medium containing 10% bovine serum.Two weeks before the addition of Fenitrothion, the cells were transformed into phenol red-free RPMI 1640 medium containing 10% charcoal/dextran treated bovine serum.There were seven groups, vehicle control group, positive group and five Fenitrothion groups.MCF-7 cells proliferation test, MTT test and growth curve were used to assess the effect on the proliferation of MCF-7 cells by fenitrothion and estrogen receptor(ER)binding test was used to analy ze the binding ability of fenitrothion with ER.
Results Fenitrothion could induce the proliferation of MCF-7 cells at 10^-7 mol/L~10^-5 mol/L with dose-effect relationship, and this effect was significantly different from the vehicle control(P < 0.05 orP < 0.01).Fenitrothion could bind with ER and inhibit the bind of¹²⁵I-E₂ with ER.
Conclusion Fenitrothion has environmental estr ogenic activity which was mediated by ER.

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