Abstract:
Objective To explore the effect of exposure of benzene on DNA and RNA damage of lymphocytes in mice.
Methods The single cell gel electrophoresis(SCGE)assay and the assay of radioactive compounds(3H-thymidine and 14C-U ridine)incor poration were used to detect the effect on DNA and RNA damage of peripheral blood, spleen and thymic lymphocytes in mice were intraperitoneally injected with benzene at various doses.
Results Except for the 100 mg/kg dose, the rate of cells DNA with tail and the mean values of DNA tail length of peripheral blood lymphoeytes by exposure to benzene in mice were significantly higher than that of the control group, the3H-thymidine and 14C-U ridin incorporation disin-tegrations per minute(dpm)of DNA and RNA by exposure to benzene in mice were significantly lower than that of the control group.
Conclusion Benzene could cause DNA damage of peripheral blood lymphoeytes and significantly affect DNA and RNA synthesis of spleen and thymiclymphocytes in mice.