维生素E对大鼠抗氧化能力及DNA损伤影响
Effect of vitamin E on antioxidative activity and DNA damage in rats
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摘要:目的 观察补充维生素E(VE)对大鼠抗氧化能力及DNA氧化损伤的影响。方法 将42只健康初断乳Wistar大鼠, 随机分为对照组、S1、S2组。各组饲料VE含量分别为24.18, 70.83, 114.04 mg/kg, 实验期为8周。实验结束后采集血样, 分别采用荧光法测定血浆VE水平, 采用试剂盒检测血浆谷胱甘肽过氧化物酶(GSH-Px)及丙二醛(MDA)含量, 并通过单细胞凝胶电泳法检测淋巴细胞DNA氧化损伤情况。结果 S1、S2组血浆VE含量较对照组明显升高(P < 0.01);S1、S2组血浆GSH-Px和MDA含量与对照组间差异无统计学意义; VE干预各组淋巴细胞DNA自发损伤无明显差别, 在5, 10, 25μmol/L的H2O2作用下, 各组损伤均明显加重, 但组间差异无统计学意义(P > 0.05)。结论 本实验补充剂量的VE对于健康幼年大鼠血浆GSH-Px及脂质过氧化产物MDA的含量无显著影响; 对淋巴细胞DNA自发损伤及不同浓度H2O2诱导的DNA氧化损伤, 单纯补充VE亦未表现出明显的保护作用。Abstract:Objective To investigate the effect of vitamin E supplementation on antioxidative acivity and DNA oxidative damage in rats.Methods 42 weaning Wistar rats were randomly divided into 3 groups including control group, S1 and S2 groups.They were fed with fully compounded fodder for 8 weeks.The content of vitamin E in each group diet was separately 24.18, 70.83, 114.04 mg/kg.Whole blood was collected by the end of the trial.The level of plasma VE was analyzed by fluorescene spectrometry.Plasma glutathione peroxidase(GSH-Px)and malondialdehyde(MDA)were analyzed by kits.DNA damage were analyzed by single-cell gel electrophoresis(SCGE).Results The levels of plasma VE in S1 and S2 groups were higher than that of the control group.There was no difference of plasma GSH-Px and MDA between the S1, S2 groups and the control group.Intrinsic DNA damage had no differences.Induced by 5, 10 and 25μmol/L H2O2, DNA damage were enhanced while there were still no differences among those groups(P > 0.05).Conclusion VE supplementation has no effect on the plasma GSHPx and MDA of healthy young rats and it shows no protective activity on the intrinsic and H2O2 induced DNA damage.
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