育龄妇女钙吸收与维生素D受体基因多态性
Relationship between absorption of dietary calcium and Bsm I polymorphism of VDR gene in childbearing women
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摘要:目的 在我国城市居民代表性膳食条件下, 探讨育龄妇女膳食钙吸收与维生素D受体(VDR)基因BsmI酶切多态性间的关系。方法 从300名18~23岁女大学生中筛选40名受试对象, 并通过特定引物对VDR基因扩增产物, 以限制性内切酶BsmI酶切电泳条带分布分型。3 d代表性膳食适应后, 开始为期12 d的代谢实验。研究对象所摄取的食品和饮品为城市代表性膳食, 称重(包括饮水)并采集备份样品分析测定; 采集研究对象的粪便和尿液。测定并计算出每位研究对象膳食钙的吸收率。结果 不同VDR基因BsmI酶切型别的育龄妇女膳食钙吸收率不同, 即Bb < bb, 且差异有统计学意义(P < 0.05)。结论 VDR基因Bb型别的育龄妇女膳食钙吸收率低, 应引起关注。Abstract:Objective To examine the relationship between the absorption of dietary calcium and Vitamin D3 receptor (VDR)gene Bsm I RFLPs in childbearing women.Methods Forty subjects were selected from three hundred young famale students aged 18~23 in 2001.After identifying the VDR gene FokI RFLPs, the women were given the representative diets during the 3-day adaptation period and the 12-day metaboism period.The copy food and drinking for measurement of untrients and also the complete feces each day were collected.Results As expected, the significant difference of the absorption of diet calcium between VDRBsm Igene RFLPs(bb < Bb)in chidbearing women was observed(P < 0.05).Conclusion More attention should be paid to the low calcium absorption of chidbearing women with Bb in our country.
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