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马义, 马民, 周天鸿, 霍新北. 高效氯氰菊酯对德国小蠊Ca-ATPase抑制效应[J]. 中国公共卫生, 2006, 22(9): 1080-1081. DOI: 10.11847/zgggws2006-22-09-34
引用本文: 马义, 马民, 周天鸿, 霍新北. 高效氯氰菊酯对德国小蠊Ca-ATPase抑制效应[J]. 中国公共卫生, 2006, 22(9): 1080-1081. DOI: 10.11847/zgggws2006-22-09-34
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MA Yi, MA Min, ZHOU Tianhong, . Inhibition of High effect cypermethrin on Ca-ATPase of Blattella germanica[J]. Chinese Journal of Public Health, 2006, 22(9): 1080-1081. DOI: 10.11847/zgggws2006-22-09-34
Citation: MA Yi, MA Min, ZHOU Tianhong, . Inhibition of High effect cypermethrin on Ca-ATPase of Blattella germanica[J]. Chinese Journal of Public Health, 2006, 22(9): 1080-1081. DOI: 10.11847/zgggws2006-22-09-34
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高效氯氰菊酯对德国小蠊Ca-ATPase抑制效应

Inhibition of High effect cypermethrin on Ca-ATPase of Blattella germanica

    摘要
  • 摘要:
      目的   研究高效氯氰菊酯与德国小蠊Ca-ATPase活性水平的量效与时效关系, 进一步完善高效氯氰菊酯作用机制的研究。
      方法   参照国标GB 13917.1-13917.8 92《农药登记卫生杀虫剂室内药效试验方法》喷施一定剂量不同浓度的高效氯氰菊酯, 用三磷酸腺苷酶(ATPase)试剂盒测定德国小蠊Ca-ATPase活性。
      结果   48 h内高效氯氰菊酯对德国小蠊Ca-ATPase活性的抑制作用呈浓度梯度效应, 高效氯氰菊酯的浓度与德国小蠊72 h的死亡率和144 h内对Ca-ATPase活性的抑制时效呈正相关。
      结论   Ca-ATPase是高效氯氰菊酯的作用靶标之一, Ca-ATPase敏感度降低而产生的靶标抗性可能是德国小蠊对高效氯氰菊酯产生抗药性的重要原因之一。

     

    Abstract:
      Objective   To more perfectly undestand the operational mechanisms of high effect cyper methrin by researching the dosage effect and the time effect between high effect cypermethr in and Ca-ATPase activities of Blattella germanica.
      Methods   Quantitative high effect cypermethr in of different concentration was sprayed upon the method of GB 13917.1-13917.8-92 for insecticide registered testing indoo reffect.The activities of Ca-ATPase were determined by using ATPase Kit.
      Results   Inhibition of high effect cyper methrin on Ca-ATPase activities of Blattella germanica showed concentration gradient effectin 48 hours.The results showed the correlation of concentration of high effect cypermethr in and inhibiting time effect on Ca-ATPase activities of Blattella germanica in 144 hours and death rate of Blattella germanica in 72 hours was positive relationship.
      Conclusion   Ca-ATPase is one of the targets of high effect cypermethrin.Because of sensitivity decrease of Na-K-ATPase, tar get resistance may be one of the important resistant mechanisms to high effect cypermethrin.

     

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