北京地区流行性腮腺炎病毒流行株基因型分析

Analysis on genotype of mumps virus strain circulating in Beijing city

摘要:
目的了解北京地区流行性腮腺炎病毒流行株基因型分布和变异情况。
方法通过SH基因的序列分析, 与其他基因型参考株进行同源性比较, 构建亲缘进化树。
结果 17株病毒均属于F基因型, VAC-S79属于A基因型, 各流行株间核苷酸最大差异为5.4%, 与F基因型代表株Z77158(兰州)、Z77160(上海)核苷酸最大差异分别为4.8%和7.0%;与疫苗株的核苷酸差异为14.6%~15.9%。
结论近2年北京地区流行的优势株为F基因型, 未发现基因型间变异, 但存在较大的型内差异, 有变异增大趋势, 需进行持续的分子流行病学监测。

Abstract:
Objective To understand geographic distribution of mumps virus genotypes and variation of circulating mumps virus strains in Beijing.
Methods SH genes were analyzed.Genetic homology between the Beijing strains and other reference mumps strains were analyzed.
Results 17 strains were all genotype F, and VAC-S79 was genotype A.The circulating viruses showed 5.4% sequence divergence, and 4.8% sequence divergence from Z77.58(Lanzhou); 7.0% from Z77.60 (Shanghai); 14.6%~15.9% from VAC-s79.
Conclusion Genotype F is the main genotype of circulating strains in Beijing.Although all the circulating strains have genotype F, there are some variations between them.As the rate of variation is tend to increase, it seems necessary to follow up the molecular epidemiology.