大豆蛋白对大鼠血浆胆固醇影响及作用机制

赵秀娟; 贾莉; 牛玉存; 张宇秋

doi:10.11847/zgggws2006-22-09-51

大豆蛋白对大鼠血浆胆固醇影响及作用机制

Effect of soybean peptide intake on catabolism of cholesterol in rats

摘要

摘要:
目的探讨膳食大豆蛋白对高胆固醇模型大鼠血浆胆固醇及其相关血脂指标的影响及可能的作用机制。
方法 4周龄断乳雄性Wistar大鼠, 诱导高胆固醇模型28 d后, 按体重和血浆总胆固醇浓度均衡分为2组, 分别喂饲含酪蛋白和大豆蛋白的纯合成高脂饲料56 d。
结果酪蛋白组和大豆蛋白组大鼠TC、甘油三酯(TG)、载脂蛋白B(apoB)含量显著降低, 粪胆汁酸排泄量增高。但对高密度脂蛋白胆固醇(HDL-C), 载脂蛋白AⅠ(apoA-Ⅰ)水平无影响; 同时可见, 大豆蛋白可以增强大鼠肝脏3-羟基-3-甲酰基CoA(HMG-CoA)还原酶及低密度脂蛋白受体(LDL-R)基因的表达。
结论大豆蛋白可通过影响apoB、HMG-CoA还原酶及LDL-R基因的表达来改变血浆胆固醇水平, 其作用机制还需进一步探讨。

Abstract:
Objective To investigate the effects of dietary soybean pr otein intake on the concentration of serum lipids in hypercholesterolemic rats and to study the mechanisms of them.
Methods Following 28 days of inducing the hyperc-holesterolemia model successfully, rats were fed a purified diet containing either casein or soybean protein.After the experimental diets had been fed for 56 days, fasting animals were killed.
Results Comparing with the rats in casein group, the total cholesterol(TC), trig lyceride(TG)and apolipoprotein B(apoB)in soybean protein group were significantly low(P < 0.05).The concentrations of fecal excretion of bile acids were higher in the rats of soybean protein group(P < 0.05).Whereas, the changes of high density lipoprotein cholesterol, apolipopr otein AI(apoA-Ⅰ)were not significant in these rats.The levels of 3-hydroxy-3-methylglutaryl coenzyme A(HMG-CoA)reductase mRNAand low density lipoprotein receptor mRNA in soybean protein rats were significantly high.
Conclusion Dietary soybean protein intake may lower TC.Further resear ches are needed for study.

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