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宾晓农, 谭敏, 吕嘉春, 蒋义国, 陈家堃. 基因芯片筛选BPDE转化16HBE相关基因[J]. 中国公共卫生, 2006, 22(11): 1350-1352. DOI: 10.11847/zgggws2006-22-11-39
引用本文: 宾晓农, 谭敏, 吕嘉春, 蒋义国, 陈家堃. 基因芯片筛选BPDE转化16HBE相关基因[J]. 中国公共卫生, 2006, 22(11): 1350-1352. DOI: 10.11847/zgggws2006-22-11-39
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BIN Xiaonong, TAN Min, LU Jiachun, . Gene chip screen for gene expression pattern in 16HBE cells treated with BPDE[J]. Chinese Journal of Public Health, 2006, 22(11): 1350-1352. DOI: 10.11847/zgggws2006-22-11-39
Citation: BIN Xiaonong, TAN Min, LU Jiachun, . Gene chip screen for gene expression pattern in 16HBE cells treated with BPDE[J]. Chinese Journal of Public Health, 2006, 22(11): 1350-1352. DOI: 10.11847/zgggws2006-22-11-39
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基因芯片筛选BPDE转化16HBE相关基因

Gene chip screen for gene expression pattern in 16HBE cells treated with BPDE

    摘要
  • 摘要:
      目的   采用基因芯片技术筛选二羟环氧苯并芘(BPDE)转化的人支气管上皮细胞(16HBE)相关基因, 探讨该技术在分子毒理学研究中的应用.
      方法   将实验组(BPDE-16HBE)和对照组(16HBE)的mRNA逆转录合成cDNA掺入荧光分子为探针, 杂交于H40S基因芯片, 分析2组基因的差异表达.
      结果   在4096种人类基因中, BPDE转化的16HBE和正常16HBE间存在差异表达的基因有143条, 其中高表达52条, 低表达91条.
      结论   基因芯片技术在筛选BPDE转化的16HBE相关基因改变上, 具有高通量、高敏感、快速等特点, 在毒物的分子致癌机制研究中意义重大.

     

    Abstract:
      Objective   To screen the differentially expressed genes in human bronchial epithelial cell line(16 HBE)after treatment with dihydroxyepoxy benzo pyrene(BPDE)by cDNAmicroarray.
      Methods   16 HBEcells and 16 HBEcells were treated with BPDEonly.The total RNAs were extracted from these cells.The cDNAprobes were prepared by labeling with Cy3-dCTPand Cy5-dCTPrespectively through reverse transcription.The mixed probes were then hybridized to the cDNAmicroarray chips containing 4096 human genes.The chips were scanned by ScanArray 4000 laser scanner.The acquired fluorescent signals were analyzed by GenPix Pro 3.0 software.Bioinformatical analysis of those differentially expressed genes had been performed.
      Results   143 genes exhibited differential expression between cells treated with BPDEonly and 16 HBE.The expression of 91 genes(63.63%)was down-regulated and of 52 genes(36.36%)was up-regulated.
      Conclusion   The regulation of genes including stress response genes, immune related genes, DNAsynthesis and repair genes Metabolism genes may be involved in itransforming activity of BPDE.

     

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