宁波地区甲型副伤寒沙门菌基因分型
Analysis on gene typing of Salmonella paratyphi A in Ningbo city
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摘要:目的 了解宁波地区甲型副伤寒沙门菌的脉冲场凝胶电泳(PFGE)基因型和流行优势型, 为探讨传播途径和追踪传染源等提供依据.方法 用全自动细菌鉴定仪(Vitek32)确定细菌的生物学特征; 用诊断血清确定细菌的血清型; 用PFGE方法对细菌的染色体进行基因分型.结果 102株甲型副伤寒沙门菌可分成9个PFGE型, 其中X2型73株, 占71.57%, X1型21株, 占20.58%, X7型2株, 占1.96%, 其他6个型各1株, 分别占0.98%.其中优势型为X2型, 菌株的带型变化在l~3条范围内, 表明宁波地区的甲型副伤寒沙门菌变异不大, 有可能是同一克隆系的甲型副伤寒沙门菌.结论 PFGE分型对探讨伤寒、副伤寒疫情内在联系具有重要意义, 该法特异性高、重复性好、结果容易判读, 有实际应用价值.Abstract:Objective To investigate the dominant gene type of Salmonella paratyphi Ain Ningbo bypulsed-field gelelectrophoresis(PFGE), and to provide a way to distinguish the causing pathogens in the epido miologic investigations.Methods The biolog ical character istics and sero-types were determined by Vitek32 and serolog ical test, respectively.Chromosomal gene patterns were typed by PFGE.Results 102 strains salmonella p ar atyp hi Awere divided into 9 PFGEtypes: 73 strians in X2 type(accounting for 71.57%), 21 in X1 type(20.58%), 2 in X7 type(1.96%), while 1 in the each other type(0.9%, respectively).The dominant type was X2.The PFGEDNAfingerpr ints differed among 1 to 3 bands, which demonstr ated that little mutat ion existed in Salmonella paratyphi Astrains in Ningbo, suggesting that all stratus maybe belong to the same clone of Sal monella paratyphiA.Conclusion PFGEtyping plays a vital role in surveillance for and the investigation of the outbreaks of Sal monella par atyp hi Afor its good speciality and reproducibility and easiness to judge.
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