Abstract:
Objective To investigate the cell growth and cell apoptosis of Lycium barbarum polysaccharides(LBP) treated on human cevical carcinoma Hela cell cultured in vitro.
Methods Hela cells were treated with different doses of LBP, the rate of cell growth was detected by using trypan blue exclusion staining, and taking MTT assay to analyze the inhibition of cell growth, then the rate of cell apoptosis was measured by flow cytometry.
Results LBP could increasingly inhibit the growth of cervical carcinoma Hela cell line with dose2effect.The biggest inhibition rate was up to 96.85% in those treated with LBP, and the apoptosis rate was 36.8%.
Conclusion LBP could inhibit the growth of Hela cell line in vitro, and the mechanism might be related to the influence of LBP in the cell cycle and the cell apoptosis induced by LBP.