挥发性有机化合物对p53、rasp21、p16表达影响

Study on p53, rasp21 and p16 expression in NIH/3T3 cells treated by volatile organic compounds

摘要: 目的探讨苯、甲醛混合物对NIH/3T3细胞中p53、rasp21、p16表达的影响。方法采用免疫组化法(SABC)观察甲醛、苯混合物致NIH/3T3细胞p53、rasp21、p16的表达情况。结果经苯、甲醛混合物处理后的第9~15d,p53、rasp21、p16表达阳性率分别为26%~45%,50%~60%,阳性率远远高于阴性对照组,差异有统计学意义(P<0.01);p16的阳性率为64%~40%,阳性率低于阴性对照组,差异有统计学意义(P<0.01)。结论(VOCs)可使突变型的p53蛋白表达率增高;上调ras癌基因家族的表达产物rasp21;下调肿瘤抑制基因p16的表达。这可能是VOCs致癌的原因之一。

Abstract: ObjectiveTo study the expression of p 53、rasp 21、p 16 in NIH/3T3 cell treated by VOCs(the compounds of benzene and formaldehyde).MethodsUsing SABC methods of immunohistochemistry to observe the expression of p 53、rasp 21、p 16 in NIH/3T3 cell.ResultsThe expression of p 53 and rasp 21 increased in VOCs groups compared with negative control group(P<0.01),but the deletion of p 16 increased compared with negative control group(P<0.01).The positive rate of p 53 and rasp 21 were 26%~45% and 50%~60%,respectively.The positive rate of p 16 was 64%~40%.ConclusionOver expression of p 53,p21 and deletion of p 16 maybe one of the major reason in the occurrence of neoplasm caused by VOCs.