Abstract: ObjectiveTo explore the effects of hypoxia on the environment of spermatogenesis in rat.MethodsAdult male Wistar rats were randomly divided into four groups:control group raised at sea level;5,15 and 30 d hypoxic group raised in a hypobaric chamber stimulating 5 000 m altitude for 5,15 and 30 days respectively.Lactate dehydrogenase C4/lactate dehydrogenase(LDH-C4/LDH),gamma-glutamyl transferase(GGT),fructose and malondialdehyde(MDA)were measured in testis.ResultsLDH-C4/LDH in hypoxic 30 d group was significantly lower than that in control group(P<0.05).Activity of GGT in hypoxic 30 d group was significantly higher than that in control group(P<0.05).Fructose level in testis was not significantly changed compared with control group.MDA decreased significantly when exposed to hypoxia (P<0.05).ConclusionHypoxia changes the environment of spermatogenesis in rat.