Abstract: Objective To investigate the autoantibody responses to 9 HCC-associated antigens which were identified using SERES(serolgical analysis of recombinant cDNA expression library),and further evaluate whether or not these identified antigens can be used as markers in the detection of HCC.Methods 9 recombinant proteins were expressed from each corresponding cDNA clones which were isolated in previous study,and used as antigens to detect antibodies against each protein in sera from patients with HCC,chronic hepatitis and normal human individuals.Results The fr equency of autoantibody against 5 HCC-associated antigens ker at in23(KRT23),transcript variant 1,a2-HS gly coprotein(AHSG),ribosomal protein L17 (RRl17)、ferritin,light poly teptide(FTL)、DEAD(Asp-Glu-Ala-Asp)box polypeptide 41(DDX41)were 46.4%,81.8%,78.2%,78.2%,91.0% in HCC 60.0%,53.3%,76.7%,56.7%,96.7% in chronic hepatit is,43.3%,46.7%,33.3%,43.3%,76.7% in normal human sera,respectively.There was significant difference in HCC,chronic hepatitis and normal human at KRT23,AHSG,RPL17,FTL,DDX41;The significant differcene existed between HCC and merging rate of two the other ones in KRT23,AHSG,FTL.A fter analyzing the other antigens such as osteopontin,pyruvate dehydrogenase kinase (PDHK),isoenzyme 2,activator of heat shock 90kDa protein ATP ase,selenoprotein P,plasma,1(SEPP1),no significat difference was foud in HCC,chronic hepatitis and normal human.Conclusion The 3 HCC-associated ant igens such as KRT23,AHSG,FTL may be one of potential markers in HCC early detection.