Abstract: Objective To study the effect of diffierent amino acid(AA)solutions on growth of multiple cellular spheroid (MCS)of ovarian cancer.Methods Different AA were applied to MCS,including balance AA,arginine-enriched,methionine-depleted,valine-depleted and complex AA which was composed of arginine-enriched,methionine-depleted and valine -depleted.Observe and analyze growth curve and cell cycle,and detect expression of proliferation cell nuclear antigen(PCNA).Results MCS in balance AA grew most fast,while MCS in complex AA grew most slowly,which was significantly different from other groups(P<0.05).Most of the cells in balance AA were in S phase,the number of cells in G0/G1 phase was(41.5±6.3)%.Most of the cells in complex AA were in G0/G1 phase(77.6±4.7)%,which was signficantly different from other groups(P<0.05).Expression of PCNA of cells in complex AA was lower than other groups.Conclusion Balance AA can stimulate MCS to grow.The four kinds of imbalance AA can inhibit MCS and block them into proliferative phase,and complex AA has the most significant effect.