DHA复合物对小鼠H22肝癌细胞TPK活性影响

Effects of docosahexaenoic acid compound on activity of TPK in H22 cancer cells of mice

摘要: 目的研究二十二碳六烯酸(DHA)复合物抗癌作用机制.方法以移植H₂₂肝癌细胞的小鼠为模型,利用γ-³²P三磷酸腺苷(ATP)作为反应启动剂掺入外源性底物的方法测定酪氨酸蛋白激酶(TPK)活性,观察DHA复合物对H₂₂癌细胞内TPK活性的影响.结果 DHA复合物的体内抑瘤率可高达70%以上;癌细胞细胞核、细胞质、细胞膜、内质网、线粒体中TPK的活性普遍升高(P<0.01).给予DHA复合物作用后,癌细胞上述各组分中TPK的活性均显著下降(P<0.01).结论 DHA复合物具有抑制TPK活性,降低细胞内的信号传递,抑制癌细胞增殖的作用.

Abstract: Objective To study the anti-tumor mechanism of DHA compound.Methods The transplanted H₂₂ liver cancer mouse model was used to explore the effect of DHA compound on the activity of tyrosine protein kinase(TP K)in H₂₂cancer cells by using the incorporation ofγ-³² Padenosine triphosphate(ATP)into exogenous substract.Results The antitumor rate of DHA compound was more than 70%.The activity of TP K of nucleolus,cytoplasm membrane,endoplasmic reticulum and mitochondria was elevated significantly in H₂₂ cancer cells(P<0.01),but it decreased obviously after being treated with DHA compound(P<0.01).Conclusion DHA compound could inhibit the activity of TP K and decrease signal conduction with cancer cells.