Abstract: Objective To investigate health education of AIDS knowledge of the medical college students to provide scientific evidence for making out the strategy of health education and synthetic prevention measure purposely.Methods An anonymous questionnaire was conducted to investigate the medical college students.the software Epidata 3.02 and SAS8.0 were used to perform the statistical analysis of the data.Results 13.74% of medical university students were satisfied with the health education of AIDS know ledge held in the school,and 28.65% were not.46.78% of the students thoug ht their AIDS prevention knowledge was not enough,and 9.60% though tenough.Their AIDS knowledge came from mass media priority,preference books and school education 22.50% of the students thought that their schools never hold any activities about AIDS prevention.Conclusion Many universities don't think much of AIDS prevention education;the situation of the demand of AIDS knowledge of medical college student is unfit to the school education.The college students should be educated intensively on the health knowledge of AIDS.