Abstract:
Objective To study the effects of PM
2.5 collected in duststorm and normal weather days on the secretion of interleukin-8(IL-8)and tumor necrosis facto-rA(TNF-α)human lung adenocarcinoma A549 cells(A 549 cells).
Methods PM
2.5 was collected during duststorm and normal weather days in Wuwei,a city of Gansu province.A549 cells were exposed to PM
2.5 at the doses of 25,50,100,200 μg/ml,respectively.After 24 hours,cytotoxicity of the PM
2.5 was measured by cell number counting.The levels of IL-8 and TNF-α were measured using radio-immunity assay.
Results PM
2.5 collected in duststorm increased the proliferation of A549 cells at low doses while inhibited it athigh doses.PM
2.5 collected in duststorm and normal weather days,however,inhibited at the tested concentrations.Both kind of PM
2.5 increased the secretion of IL-8.However,there were no significant changes in the secretion of TNF-α from cells treated by both kind of PM
2.5.
Conclusion PM
2.5 from the sandstorm dust and non-sandstorm dust could induce the early inflammatory reaction in the A 549 cells.