Abstract: Objective To obtain and construct eukaryotic expression vector of hBD₂.To examine the expression of hBD₂ in L929 and observe the antimicrobial activity of recombinant hBD₂.Methods The total RNA was isolated from human cervical carcinoma tissue and hBD₂ cDNA fragments were obtained by RT-PCR amplification with specific primers,and inserted into pCAGG vector.The expression of hBD₂ in L929 was examined by RT-PCR,the antimicrobial activity of recombinanthBD₂ was assessed by Kir by-Bauer disc agar diffusion test.Results It was proved that we successfully cloned the cDNA of hBD₂ and constructed its recombinant eukaryotic expression vector pCAGG-hBD₂.Recombinant hBD₂ in supernate showed antimicrobial activity.Conclusion Recombinant expression vector of hBD₂ could be effectively expressed in L929,and the recombinant hBD₂ in supernate showed obvious antimicrobial effects toward some standard bacteria lines.