维生素E对CC14作用下HSC增殖和ECM影响

Effect of vitamin E on proliferation of HSC injured by CCl4

摘要: 目的观察维生素E(VE)对四氯化碳(CC1₄)作用下大鼠肝星状细胞(HSC)增殖和细胞外基质(ECM)产生的影响。方法采用原位灌注方法分离大鼠HSC。结果 CC1₄作用下大鼠HSC³H-脯氨酸(³H-Pro)的掺入显著增加,Ⅲ型前胶原(PCⅢ)、透明质酸(HA)、板层素(LN)及纤维连接素(FN)的分泌水平明显提高。200nmol/LVE预处理4h可有效抑制CC1₄作用下大鼠HSC³H-胸腺密啶(³H-TdR)、³H-Pro的掺入,VE保护能使CC1₄作用下HSC分泌PCⅢ、HA、LN和FN减少与HSC产生丙二醛(MDA)下降,二者呈正相关。结论 VE可抑制CC1₄作用下大鼠HSC增殖和通过抗氧化损伤抑制HSCECM的分泌。

Abstract: Objective To study the inhibitory roles of vitamin E(VE)on cell proliferation and ex tracellular matrix (ECM)synthesis of hepatic stellate cells(HSC)injured by Carbon Tetrachloride(CCl4)in vitro.Methods Rat HSC were isolated and cultured.Results Addition of CC1₄ to HSC pretreated with 200 nmol/L VE4 hours,the incorpo ration of ³H-thymidine and ³H-proline and the secretion of precollag entype Ⅲ,hyaluronic acid,laminin and fibronectin as well as production of malodialdehyde(MDA)were obviously inhibited.The analysis of correlation indicated that the effects of VE on inhibition of ECM secretion depended on lowering the production of MDA.Conclusion VE can inhibit the cell proliferation and secretion of ECM in rat's HSC injured by CC1₄ in vitro.