Abstract: Objective To study the incidence and its risk factors of tuberculosis(TB)among the employess of the anti-TB instiutions in Henan province for the reference to establish and perfect the measures,strategies and system of the vocational biosafety of TB.Methods The cohort consisted of all employess in the anti2TB institutions of Henan from Jan 1,1995 to Dec 31,2004.All employees were interviewed to survey retrospectively their work experience,TB incidence and others.Incidence density and relative risk(R R)was used to measure vocational biosafety of TB.Cox regression model was used to do multivariate analysis.Results The incidence rates of TB,pulmonary TB and smear-psotove TB among 4 429 employees with retrospective survey were 44914/105 person-years(PY)(healthcare worker:579.1/105 PY;non-healthcare worker:20518/ 105PY),38811/105 and 13612/105 PY respectively.The OR value of healthcare worker regarding non-healthcare as reference was 2.89(95%CI1.81～4.60)with multivariate cox regression model.Conclusion The TB incidence rate among employees working in the anti-TB was higher than that of non-healthcare worker in anti-TB institutions;the condition of TB vocational biology safety in anti-TB institutions,and its corresponding administrations and bio-sefety of the healthcare workers need to be improved.