高级检索
周俊英, 付有荣, 郭清莲, 齐小保. 生鲜肉中肠炎沙门菌分子生物学特征分析[J]. 中国公共卫生, 2007, 23(10): 1227-1228. DOI: 10.11847/zgggws2007-23-10-40
引用本文: 周俊英, 付有荣, 郭清莲, 齐小保. 生鲜肉中肠炎沙门菌分子生物学特征分析[J]. 中国公共卫生, 2007, 23(10): 1227-1228. DOI: 10.11847/zgggws2007-23-10-40
ZHOU Jun-ying, FU You-rong, GUO Qing-lian, . Analysis on characteristics of salmonella enteritidis from raw meat[J]. Chinese Journal of Public Health, 2007, 23(10): 1227-1228. DOI: 10.11847/zgggws2007-23-10-40
Citation: ZHOU Jun-ying, FU You-rong, GUO Qing-lian, . Analysis on characteristics of salmonella enteritidis from raw meat[J]. Chinese Journal of Public Health, 2007, 23(10): 1227-1228. DOI: 10.11847/zgggws2007-23-10-40

生鲜肉中肠炎沙门菌分子生物学特征分析

Analysis on characteristics of salmonella enteritidis from raw meat

  • 摘要: 目的 了解生鲜肉类中肠炎沙门菌耐药性及基因变异情况。方法 药敏试验采用K-B法,用碱裂解法提取细菌的质粒DNA,采用肠杆菌重复基因间隔重复共有序列(ERIC-PCR)技术对10株肠炎沙门菌进行基因分析,运用NTSYS-PC2.0软件进行同源性比较,聚类分析。结果 药敏呈现出多重耐药;基因谱系与聚类分析可将菌株分为3大类,扩增片段长度为250bp~2000bp,在85%的同源度上可分为A、B、C等3个型,其中A型2株,B型6株,C型2株。结论 生鲜肉中肠炎沙门菌耐药情况比较普遍,10株肠炎沙门菌基因变异较小,ERIC-PCR技术是分析、鉴定肠炎沙门菌,追溯食物中肠炎沙门菌的有用和可行的工具。

     

    Abstract: Objective To assess the antibio tics susceptibility and genetical diversity of salmonella enteritidis isolated from raw and fresh meats.Methods Gene analysis by antibiotis susceptibility,ERIC-PCR techniquea and plasimid fingerprinting were put into practice for the detection of gene variation of 10 strains of salmnoella enteritidis.The similar ities between different strains were obtained by software NTSYS-PS210 for cluster analysis.Results 10 strains of salmonella enteritidis appear mult-i antibiotics resistance.These strains were subtyped into 3 groups A,B,C according to the electrophoresis profiles sinilarity 85% standard or above,including 2 strains,6 strains and 2 strains respectively.Conclusion These strains exist wide antibiotics resistance,10 strains of Salmonella enteritidis have lower genetical diverity.ERIC-PCR is a powerful tool for differentiating salmonella enteritdis and tracking the transmission of salmonella enteritidis in food.

     

/

返回文章
返回