Abstract:
Objective To assess the antibio tics susceptibility and genetical diversity of
salmonella enteritidis isolated from raw and fresh meats.
Methods Gene analysis by antibiotis susceptibility,ERIC-PCR techniquea and plasimid fingerprinting were put into practice for the detection of gene variation of 10 strains of salmnoella enteritidis.The similar ities between different strains were obtained by software NTSYS-PS210 for cluster analysis.
Results 10 strains of
salmonella enteritidis appear mult-i antibiotics resistance.These strains were subtyped into 3 groups A,B,C according to the electrophoresis profiles sinilarity 85% standard or above,including 2 strains,6 strains and 2 strains respectively.
Conclusion These strains exist wide antibiotics resistance,10 strains of
Salmonella enteritidis have lower genetical diverity.ERIC-PCR is a powerful tool for differentiating
salmonella enteritdis and tracking the transmission of
salmonella enteritidis in food.