Abstract: ObjectiveTo explore the effect of IFN-α2b on inducement to CD25 and its mRNA in the patients with chronic hepatitis B.MethodsThe 160 typical chronic hepatitis Bpatients,including in light 93 and in moderate 67,were treated with IFN-α2b and routine medicine,and the expression of CD25 and its mRNA before and after being stimulated with PHA was dynamically detected by biotin-streptavidin(BSA)and Real-time PCR,respectively.ResultsThe expression of CD25 and its mRNA could be increased after treatment of IFN-α2b.After the third course of treatment,there was significant differ ence between in the subgroup of IFN and in the subgroup of routine medicine(P<0.05).In our study,as if the expression of CD25 mRNA was paralleled with that of CD25.ConclusionThe low expression of CD25 and CD25 mRNA was identified in the patients with chronic hepatitis B and associated with activity of pathog enetic condition.The expression of CD25 and CD25 mRNA can be induced by IF N-A2b so that a lot of active Tlymphocytes being activated,and the cellular immune function of host can be improved after treatment obviously.