塞来希布对胃癌细胞增殖抑制作用

聂海祺; 罗姚佳; 文涛; 孙黎光; 李剑平

doi:10.11847/zgggws2008-24-05-06

塞来希布对胃癌细胞增殖抑制作用

Approach of gastric carcinoma cells proliferation inhibited by celecoxib

摘要

摘要: 目的观察塞来希布对碱性成纤维细胞生长因子(bFGF)促进胃癌BGC-823细胞的增殖抑制影响,探讨塞来希布抑制肿瘤细胞增殖的机制.方法不同浓度组的塞来希布作用于经25 ng/ml bFGF作用后的胃癌BGC-823细胞,用蛋白印迹(western blot)分析法检测环氧化酶-2(COX-2)、核转录因子-κB(NF-κB)和血管内皮生长因子(VEGF)蛋白的表达变化.结果 25 ng/ml bFGF通过PKB信号转导通路能增强胃癌BGC-823细胞中COX-2、NF-κB、VEGF蛋白的表达,对胃癌BGC-823细胞有促增殖作用,呈时间依赖性(P<0.01).不同浓度组的塞来希布对经25 ng/ml bFGF作用的胃癌BGC-823细胞中COX-2、NF-κB、VEGF蛋白的表达均有抑制作用(P<0.01).结论塞来希布对bFGF促进BGC-823胃癌细胞的增殖有一定的抑制作用.

Abstract: Objective To observe the depression effects induced by celecoxib on gastric carcinoma BGC-823 cells proliferation induced by bFGF,to investigate the mechanism of inhibition induced by celecoxib on tumour cell proliferation.Methods After treatment of different dosages of celecoxib,expressions of COX-2,NF-B,VEGF protein in gastric carcinoma BGC-823 cells induced by 25 ng/mlbFGF were measured using western blotting.Results 25 ng/ml bFGF promoted BGC -823 cell proliferation and expressions of COX-2,NF-B,VEGF protein in a time-dependence way by PKB signal transduction(P<0.01).Celecoxib with different concentrations had proliferation-inhibiting effect on BGC-823 cell and induced apoptosis.Different dosages of celecox ib had depressant effects on the expressions of COX-2,N F-B,VEGF protein in BGC-823 cell induced by 25 ng/ml bFGF(P<0.01).Conclusion celecoxib had determinate depressant effects on gastric carcinoma BGC-823 cells proliferation induced by bF GF.

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