Abstract: Objective To investigate the kidney protection effect of soybean isoflavone(SI)in impaired glucose tolerance(I GT)rats induced by D-galactose(D-ga1).Methods To make the IGT model rat by intr aper itoneal injection(i.p.)Dgal for 8w.Two doses of SI were given intragastrically(i.g.)for 6wsince the 3rd w,and metformin as positive control.2hBG,BU N,M AU,SOD,GSH-Px,M DA and AGEs contents of kidney,DNA damage degree of renal cells in every group were tested at the end of the ex periment.Results Compared with the normal control group,2hBG,M AU,M DA and A GEs contents and renal cell DNA tailing rate o f the model group incr eased no tably(P<0.05)and SO D activity decreased signifi cantly(P<0.05).After SI interv ening for 6w,2hBG,MA U,M DA and AG Es contents and renal cell DNA tailing rate de creased obviously than model group's(P<0.05),and SOD activity increased significantly(P<0.05).AG Es co ntent of two SI groups was lo wer obviously than metformin group(P<0.05).Conclusion The IG T rat model induced by D-gal has slig ht kidney damage.SI could exert kidney protection effect by improving IGT,antioxidation and inhibition the for mation of MDA and AG Es.SI wor ks better than metformin in inhibiting glycosylation.