Abstract:
ObjectiveTo compare cytotoxic effects of microcystin-L R(MCLR)on human hepatoma cell lines HT17 and Hep G2,and then study the oxidative DNA damages induced by MCLR.
MethodsCytotoxicity was measured by MTT assay,and oxidative DNA damages were detected using immunostaining of 8-hydroxydeoxiguanosine(8-OHdG).
ResultsSignificant cytotoxic effects in HT17 cells were observed at MCLR concentrations higher than 1μg/ml.Dose-respond relationship was found between MCLR exposure and cell viability.HT17 cells were more sensitive than Hep G2 cells.MCLR even at non-cytotoxic concentration significantly increased the levels of 8-OHdG.
ConclusionHT17 cells with the expression of organic anion transporting polypeptides(OATP)1B1 are more sensitive to MCLR.MCLR even at non-cytotoxic dose induces oxidative DNA damages,which may contribute to their genotoxicity during long-term exposure.