Abstract: ObjectiveTo study the effects of Fuzhengjiedu decoction(FJD)on the genotoxicity induced by nickel sulfate (NiSO₄).MethodsBy using 16HBE cell in vitro with ³H-TdR incorporation assay techniques,the effects of FJD on nickel induced unscheduled DNA synthesis(UDS)were observed.ResultsThe UDS induced by NiSO₄(100~200 μmol/L)in 16HBE cells increased significantly compared with negative control(P<0.05,P<0.01).FJD could not induce UDS.In cells co-treated with various doses of FJD and NiSO₄(200 μmol/L)the UDS levels were lower than that of induced by NiSO₄ (CPM=998.63±81.36)(P<0.05,P<0.01).FJD could inhibit the UDS induced by NiSO₄ in dose-dependent manner (r=0.837,P<0.05).ConclusionFJD were not mutagenic and could have inhibitory effects on the genotoxicity and carcinogenicity of nickel.