烧伤脓毒症患者金黄色葡萄球菌B型肠毒素检测
Detection of staphylococcus aureus enterotoxin B in burn infection by double sandwich enzyme-linked immunosorbent assay
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摘要: 目的建立一种敏感、快速的酶联免疫测定方法,用于定量检测烧伤脓毒症患者金黄色葡萄球菌B型肠毒素的含量。方法采用生物素-链霉素系统放大的双抗体夹心酶联免疫吸附方法,以兔抗葡萄球菌肠毒素B(SEB)多克隆抗体做为包被抗体,以生物素标记的抗SEB单克隆抗体作为第2抗体进行测定。结果在0.078~10.000μg/L的浓度范围内,SEB标准品浓度与吸光度值线性关系良好,相关系数为r=0.9876;重度烧伤患者血清中SEB水平明显高于对照组及轻度烧伤组,差异有统计学意义(P<0.05)。结论双抗体夹心ELISA法检测SEB,灵敏性达0.078μg/L,可用于临床烧伤患者血清中的SEB监测。Abstract: ObjectiveTo establish a two site antibody enzyme-linked immunosorbent assay(ELISA)for detecting SEBin burn infection patients.MethodsA monoclonal antibody to staphylococcus aureus B(SEB)was used as indicator antibody.The sensitivity of ELISA was improved by biotin binding system.ResultsCalibration curves for SEB were linear from 0.078~10.000 μg/L(r=0.9876).Data showed that SEB levels after the severe burn followed by staphylococcus aureus infection were significantly higher than those of normal controls and slight burn control group(P<0.001).ConclusionThe method is rapid with high sensitivity(0.078 μg/L)and could be used to detect SEB in hospital infection patients.
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