进口三文鱼单核细胞增生李斯特菌污染检测

Survey and serotyping of Listeria monocytogenes in imported Salmon

摘要: 目的对2003~2006年由北京口岸挪威进口的三文鱼进行单核细胞增生李斯特菌污染情况监控,并对分离的单核细胞增生李斯特菌进行血清分型。方法采用美国食品药品管理局颁布的检测方法对单核细胞增生李斯特菌进行分离和血清分型。结果挪威三文鱼的单核细胞增生李斯特菌的检出率为1.2%~6.3%,在2003~2006年呈下降趋势;血清分型结果显示,分离自三文鱼的单核细胞增生李斯特菌的血清型主要是1/2a、1/2b和4b型,分别占分离菌株的85%,6%和6%。结论首次对分离自挪威三文鱼的单核细胞增生李斯特菌进行血清分型,所分离的菌株中97%是引起人类李斯特菌病的菌株。

Abstract: ObjectiveInspecitng the contamination of salmon by Listeria monocytogenes and serotyping the Listeria monocytogenes isolated from Norway salmon.MethodsThe methods of FDA BAM 8th chapter 10 and chapter 11 were used for Listeria monoytogenes isolation and ser otyping.ResultsThe result showed that rates of Norway salmon contamination by Listeria monocy togenes were form 1.2% to 6.2%.The contamination rates decreased from 2003 to 2006.The serotype of the Listeria monocytogenes isolated were 1/2a,1/2b and 4b.ConclusionAbout 97% of Listeria monocytogenes isolates from salmon imported colud cause the Listeriosis in human.