Abstract: Objective To observe the effects of lowpower microwave on serum testosterone,serum cholesterol and sexual function of male mice.Methods 48 adult male mice were randomized into 3 exposure groups and 1 control group.The exposure groups were exposed to 900 MHz microwave(250μW/cm²,150μW/cm²,50μW/cm²,24 h/d,3415 d).Capture incubation period(CIP),capture times(CT),serum testosterone,serum cholesterol were used to evaluate effects of the lowpower microwave.Results CIP was significantly increased in exposure group(150μW/cm²)compared with that of the normal control(P<0.05).Serum testosterone,serum cholesterol of exposed groups were significantly decreased compared with that of the normal control(P<0.05,respectively).In microwave exposure group(250μW/cm²)serum cholesterol was significantly decreased compared with the normal control(P<0105).Conclusion Low-power microwave radiation(150μW/cm²)can influence the level of serum testosterone,and further impact the sexual function of male mice through affecting the serum cholesterol.