三羟基苯甲酸对淋巴瘤细胞凋亡及周期影响
Effect of TAD compound on apoptosis and cell cycle progression of lymphoma cells in vitro
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摘要: 目的 观察不同剂量3,4,5三羟基苯甲酸化合物(TAD)对小鼠淋巴瘤细胞株EL-4细胞的存活率、细胞凋亡及细胞周期进程的影响,探讨其对肿瘤生长抑制的机制.方法 应用四甲基偶氮噻唑蓝(MTT)比色法和流式细胞仪检测经不同浓度(3.125~25μg/ml)TAD处理的EL-4细胞存活率、细胞凋亡率及细胞周期的变化.结果 EL-4细胞经过3.125,6.25,12.5和25μg/ml TAD处理后,各观察组细胞存活率依次为(80.60±6.01)%,(75.39±4.97)%,(71.42±3.73)%和(54.96±4.57)%;与对照组100%存活率相比明显降低(P<0.01,P<0.001).细胞凋亡率依次为(4.95±1.76)%,(19.58±7.91)%,(35.85±7.01)%,(11.67±2.96)%;明显高于对照组的(0.36±0.33)%(P<0.01,P<0.001).TAD引起细胞周期进程的改变,G1期细胞百分数明显高于对照组(P<0.05,P<0.01,P<0.001),S期细胞百分数明显低于对照组(P<0.05,P<0.01,P<0.001);G2期细胞无明显变化.结论 TAD能够降低EL-4细胞存活率,诱导EL-4细胞凋亡,阻止G1期细胞向S期进程,其抑制作用可能通过G0/G1期阻滞引起.Abstract: Objective To observe the effect of 3,4,5-trihydrox ybenzoic acid dimmer(T AD)in different concentration on apoptosis and cell cycle prog ression of lymphoma EL-4 cells in v itro and ex plore its possible mechanism of tumorg rowth inhibition.Methods Survival rate was observed with M T T assay.The changes of apoptosis and cell cycle progression were measured with flowcy tometry.Results TAD of 3.125,6.25,12.5 and 25 Lg/ml could inhibit the proliferation of El-4 lymphoma cells in vitro.The survival rates of the cells treated with TAD were significantly lowerthan that of in the control(P<0.01,P<0.001).The apo ptotic percentag es of the cells treated with TAD increased signficantly as compared with those in the control(P<0.01,P<0.001).And the percentages of G0/G1 phase cells treated wit h TA D were significantly hig herthan those of in the control(P<0.05,P<0.01,P<0.001).While the percentages of Sphase cells treated with TAD were significantly lowerthan those in the control(P<0.05,P<0.01,P<0.001).The percentages of G2 phase cells did not change significantly.Conclusion The inhibiting effect of TAD on tumorgrowth could be due to cell apoptosis caused by G0/G1 phase arrest.
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