Abstract:
Objective To investigate the genetic characteristic of vivax malaria transmission-blocking vaccine candidate
Pvs48 in Chinese isolates.
Methods Blood samples were collected from patients infected with P.vivax from Yunnan epidemic areas.The parasite genome DNA was extracted.The DNA sequences were amplified.PCR products were directly sequenced.
Results The 16 sequences of
Pvs48 complete gene were obtained.Compared with
Pvs48 Sal-I sequence,there were 6 non -synonymous mutation sites,3 kinds of haplotypes and 3 amino acid substitutions.
Conclusion The limit genetic mutation of
Pvs48 suggests that
Pvs48 antigen is conserve.The limit genetic mutation of
Pvs48 reinforces that the vivax transmission -blocking vaccine base on
Pvs48 would promise a good prospect for a wide range application in China.