Abstract:
Objective To explore the relationship of inhibitory proliferative ability induced by intracellular S-adenosylhomocysteine(SAH)accumulation and the expression of est rogen receptor(ER)in human umbilical vein endothelial cells.
Methods I mmortalized HUVEC were treated without(normal)or with different concent rat ion 3-deazaadenosine (DZA),a potent S-adenosylhomocysteine hydrolase(SAHH)inhibitor,for 24,48 and 72h.The proliferative ability of HUVEC was measured with flow cytometry and TdT mediated dUTP nick ending labelling(TUNEL)methods.The ER protein and mRN A expression was analyzed with western blot and quantitative rev erse transcription-polymerase chainraction (qRT-PCR)respectively.The promoter methylation of ER gene was detected with methylation special PCR(M SP).
Results The proliferative ability was decreased(mainly arrested at G
1/G
0 phase),and the apoptotic rate was increased after HUVEC treatment with DZA(P<0.05).The relative expression of ER mRNA and protein in HUVEC treated with DZA was lower than that of normal(P<0.05),but there was no changes of ER gene promotermethylation.
Conclusion The inhibitory proliferative ability induced by intracellular SAH accumulation is correlative with ER expression,which not through altering promotermethy lation in HUVEC.