Abstract: Objective To evaluate HBeAg negative HBV infectious pregnant women with or without pre-C 1896 "stop" mutation and their mother to infant infection.Methods HBV-DNA load in blood of pregnant women was detected by real time fluorescence quantitative PCR.HBV pre-C1896 "stop" mutation of the HBV positive mother and their HBV infected infants was analyzed by mismatched PCR restricted fragment length polymorphous assay.Results The rate of HBV mother to infant infection with HBeGg negative pregnant women was 5.57%.The higher infection rate was,the higher HBV was loaded in blood of pregnant women.The infection rate of HBV high load group(≥10⁶ copies/ml)was 34.7%,which was higher than that of low load group(10⁴~10⁶ copies/ml,17.1%)(P<0.01).HBV gene of HBV infected pregnant women with "stop" mutate and their newly born babies was analyzed,and found that all of the five HBV infected infants delivered by wild strain advantage mothers were tested as wild strain HBV carriers.Among four mutant strain advantage HBV infected mothers,two of their infants were found to have mutant strain HBV infection,and the other two as wild strain infection.Conclusion The risk of mother to infant HBV infection with HBeAg negative mother is related to mother&s HBV load in the blood.For coinfected HBV pregnant women with "stop" mutant strain and wild strain,mother to infant infection of wild stain HBV occurred more easily than that of mutant strain.