缺氧对PC12细胞IL-6及GP130基因表达影响

Study on changes of IL-6/GP130 signal transduction pathway induced by hypoxia in PC12 cells

摘要: 目的研究缺氧对大鼠嗜铬细胞瘤(PC12)细胞中白细胞介素6(IL-6)和IL-6受体β亚基(GP130)表达的影响。方法制备缺氧细胞模型。采用放射免疫技术、逆转录-聚合酶链反应(RT-PCR)技术和免疫蛋白印迹(Western B lot)法检测IL-6基因mRNA和活性、GP130基因mRNA和蛋白表达水平。结果 PC12细胞在缺氧后2 hIL-6mRNA(1.121±0.076)和活性((167.27±12.03)pg/m l)、GP130mRNA(1.695±0.089)和蛋白表达(1.652±0.137)增强(P<0.05),6 h(分别为(1.431±0.115),(320.66±11.82)pg/ml,(2.134±0.141),(2.521±0.164))达到高峰(P<0.01),显著高于对照组(分别为(0.897±0.073,69.71±8.51)pg/ml,(1.356±0.082),(1.029±0.032))。结论缺氧能增强IL-6和GP130基因的表达。

Abstract: Objective To explore the effect of hypoxia on the expressions of interleuk in-6 and GP130 in PC12 cells.Methods The PC12 cells were treated with hypoxia and the mRNA and protein expressions of IL-6 and GP130 were detected by RT-PCR,Western Blot and RIA.Results The mRNA and protein expressions of IL-6 and GP130 in PC12 cell were obviously increased at 2h and reached the highest level at 6h,decreased at 12h under hypoxia condition,but they were still higher than those of the control group.Conclusion The results suggest that IL-6 and GP130 lead to important modulation in the brain damage after hypoxia injury.