腰果主要过敏原分离鉴定与纯化

Isolation, identification and purification of allergens in cashew nut

摘要: 目的对腰果的主要过敏原进行分析、鉴定与纯化。方法通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)电泳分离腰果的蛋白质组份,采用免疫印迹(Western-blotting)方法鉴定过敏原,通过离子交换层析对腰果主要过敏原进行初步纯化。结果腰果粗提液SDS-PAGE显示有10条蛋白条带,Western-blotting显示对腰果过敏患者的阳性混合血清能与其中2个蛋白条带起反应,分子量分别为21kD和11kD。离子交换层析可初步纯化出21和11kD的过敏原蛋白。结论对腰果过敏原成功进行分离和鉴定,并初步纯化出腰果的主要过敏原。

Abstract: Objective To isolate,identify and purify the allergens in cashewnut,anacard lun occidentale L.Methods The total protein of the cashew nutwas iso lated by SDS-PAGE.The allergens of cashew nutwere identified by Westernblotting.To purify the allergens,ion exchange chromatography was employed.Results There were tenprotein bands visible on the SDS-PAGE gel.The allergens with molecular mass of 21kD and 11kD were identified by Western-blotting.The 21kD and 11kD allergens of cashew nutwere purified by ion exchange chromatography.Conclusion The allergens of cashewnut were isolated and identified.And the allergens of cashewnut were purified prmiarily.