Abstract: Objective To detect physiological toxicity and genotoxicity of pharm aceutical factory waste water.Methods The biochemical indices(the red blood cells micronuclei rate,GPT,GOT) in locah treated with the write water were detected Results After 6 days of treahn ent,the red blood cell micronuclei rate in exposure group was remarkably higher that that of the control group.At the same time,the GPT and GOT activity in liver decreased with the increasing of concentration of waste water.The value of enzyma activity of the stock solution was 36.6453 and 66.3031,respectively,with reductions of 76.90% and 80.61% comparedwith the control However,the activity of the enzymes in the semm was enhanced and the highest average values of enzym a activity of the stock solution were 501.0423 and 639.1670 with the increases of 83.34% and 96.09% compared with the control.Conclusion The phaun aceutical factory wastew ater has signifcant genotoxicity and physiological toxicity on loach.