Abstract:
Objective To develop a tpyingmethod ofmultiplex PCR-DHPLC forVibrio cholerae.
Metbods The special priners were composed to expand the collagenase gene(vcc gene) of Vibrio cholerae,the virulent genes of V.cholerae O 1 and O 139(ctxA gene and tcpA gene),and the virulent gene of V.cholerae O 139 (LPSgt gene),respectively.The multiplex PCR-DHPLC typing method was developed after the PCR anneal temperature for four pairs of grin ers being optinized.
Results The fourgeneswere amplified synchronously. The typing results of V.cholerae bymultiplex PCR-DHPLC method were the same as those of anticipated.
Conclusion It is rapid and exact to identify whether the bacteria pure culture is V.cholerae and idiographic strain according to themultiplex PCR-DHPLC detection result.